Hamler exited the game with a left knee injury during the second quarter in Week 3 against the New York Jets.
Expect Denver to place Hamler on injured reserve shortly.
Hamler, 22, was a two-year starter at Penn State and earned second-team All-Big Ten honors before being selected by the Broncos in the second round of the 2020 NFL Draft.
He signed a four-year, $7,137,128 rookie contract that includes a $2,750,638 signing bonus.
In 2021, Hamler has appeared in three games for the Broncos, recording four catches for 46 yards.